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| Registros recuperados: 140 | |
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| Diaz Almela, F; Boudry, Pierre; Launey, Sophie; Bonhomme, Francois; Lapegue, Sylvie. |
| The geographical structure of 15 natural populations of the flat oyster (Ostrea edulis L.) was assessed by single-strand conformation polymorphism (SSCP) of a 313-base-pair (bp) fragment of the mitochondrial 12S-rRNA gene. Fourteen haplotypes were observed, with one being dominant in the Mediterranean samples and another one in the Atlantic populations. The geographically extreme populations sampled in Norway and the Black Sea appeared differentiated by exhibiting the dominance of a third group of haplotypes. The results were compared to available microsatellite data at five loci. The Atlantic/Mediterranean differentiation pattern was qualitatively the same with both types of markers, confirming an isolation-by-distance pattern. The average mitochondrial... |
| Tipo: Text |
Palavras-chave: Ostrea edulis; Polymorphism; Flat oyster; Population; Gene. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/publication-680.pdf |
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| Huvet, Arnaud; Boudry, Pierre; Baud, Jean-pierre; Nourry, Max; Phelipot, Pascal; Gerard, Andre; Ledu, Christophe; Heurtebise, Serge; Cornette, Florence; Blouin, Frederic; Bedier, Edouard; Lapegue, Sylvie. |
| es marqueurs génétiques, particulièrement bien adaptés à ces problématiques car hypervariables, ont été mis au point récemment dans notre laboratoire en collaboration avec le laboratoire Génome et Populations de Montpellier (CNRS). Ils permettent désormais l'analyse approfondie et la discrimination fine de populations d'huîtres creuses Crassostrea gigas et de rechercher si certaines d'entre-elles n'auraient pas des caractéristiques intéressantes pour l'ostréiculture locale (croissance, résistance estivale, affinage ... ). La diffusion de telles populations auprès de la profession serait plus rapide car il s'agit de la même espèce. Selon les recommandations du CIEM, il faut effectuer une mise en quarantaine des huîtres importées (après contrôles sanitaires)... |
| Tipo: Text |
Palavras-chave: Génétique; Génétique des populations; Marqueurs génétiques; Microsatellite; Huître creuse; Crassostrea gigas. |
| Ano: 1998 |
URL: http://archimer.ifremer.fr/doc/00044/15563/12952.pdf |
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| Boudry, Pierre; Lapegue, Sylvie; Guyomard, R.. |
| The Working Group on the Application of Genetics in Fisheries and Mariculture (WGAGFM) met in La Rochelle, France from 10-12 March, 2003. Seven persons representing six countries were present. Three terms of reference (ToR) were addressed, while one was deferred until the appropriate information was available for review. WGAGFM first considered ToR a) Review and report on the practical use of genome mapping in aquacultured organisms. Genome maps are used extensively in the production of selected strains of agriculture plants and livestock. Studies on the genome of aquatic animais have been summarised in the WG report. Genome mapping projects in aquaculture exist for most major species and linkage maps are now becoming available. WGAGFM produced four... |
| Tipo: Text |
Palavras-chave: Conservation of genetic diversity; Triploids; Recommandation; Aquaculture; Fish; Genome mapping; Genetic. |
| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/2003/acte-3301.pdf |
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| Rohfritsch, Audrey; Bierne, Nicolas; Boudry, Pierre; Heurtebise, Serge; Lapegue, Sylvie. |
| Originating from the north eastern Asia, Crassostrea gigas has been introduced and translocated, mainly for aquaculture purpose, into several European countries (from Norway to Portugal and in the Mediterranean Sea) (1). Although highly variable, the invasiveness pattern of C. gigas has been demonstrated in several countries and therefore considered as a pest or a noxious species in those areas (2 and 3).Our project aims at identifying the characteristics of such a flourishing species: can its success be explained by chance and/or global warming only or does it exhibit a more important potential of adaptation than other species? Therefore we developed a population genomics approach, known as "genome scan". It corresponds to the study of numerous loci... |
| Tipo: Text |
Palavras-chave: Invasive; AFLP; Genome scan; Crassotrea gigas; Pacific oyster. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/acte-4805.pdf |
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| Sauvage, Christopher; Bierne, N; Lapegue, Sylvie; Boudry, Pierre. |
| DNA sequence polymorphism and codon usage bias were investigated in a set of 41 nuclear loci in the Pacific oyster Crassostrea gigas. Our results revealed a very high level of DNA polymorphism in oysters, in the order of magnitude of the highest levels reported in animals to date. A total of 290 single nucleotide polymorphisms (SNPs) were detected, 76 of which being localised in exons and 214 in non-coding regions. Average density of SNPs was estimated to be one SNP every 60 bp in coding regions and one every 40 bp in non-coding regions. Non-synonymous substitutions contributed substantially to the polymorphism observed in coding regions. The non-synonymous to silent diversity ratio was 0.16 on average, which is fairly higher to the ratio reported in other... |
| Tipo: Text |
Palavras-chave: Crassostrea gigas; Codon bias; Genetic diversity; SNP. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3417.pdf |
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| Hedgecock, Denis; Launey, Sophie; Pudovkin, A. I.; Naciri, Yamama; Lapegue, Sylvie; Bonhomme, Francois. |
| The great fecundity and very high larval mortality of most marine invertebrates and fish make possible substantial variance in the number of offspring contributed by adults to subsequent generations. The reproductive success of such organisms may thus resemble a sweepstakes lottery, in which a minority of progenitors succeeds in replacing an entire population, while the majority fails to procreate. One specific prediction of this hypothesis, that genetic diversity of newly settled cohorts should be less than that of the adult population, is tested in the present study. Microsatellite DNA markers were examined in naturally spawned juvenile European flat oysters Ostrea edulis (L.), collected over a 12-day period in 1993 from the western Mediterranean Sea,... |
| Tipo: Text |
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| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-2616.pdf |
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| Lallias, Delphine; Boudry, Pierre; Lapegue, Sylvie; King, Jon W.; Beaumont, Andy R.. |
| The native European flat oyster Ostrea edulis is listed in the OSPAR Convention for the Protection of the Marine Environment of the North-East Atlantic (species and habitat protection) and in the UK Biodiversity Action Plan. Once extremely abundant in the 19th century, European stocks of O. edulis have declined during the 20th century to rare, small, localised populations due to overexploitation, habitat degradation and, most recently, the parasitic disease bonamiosis. Selective breeding programmes for resistance to bonamiosis have been initiated in France and Ireland. High genetic diversity and bonamiosis-resistance would be important features of any sustainable restoration programmes for O. edulis. Oysters were sampled across Europe from four hatchery... |
| Tipo: Text |
Palavras-chave: Restoration programme; Ostrea edulis; Genetic variation; Pedigree reconstruction. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00001/11259/11375.pdf |
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| Bechemin, Christian; Soletchnik, Patrick; Polsenaere, Pierre; Le Moine, Olivier; Pernet, Fabrice; Protat, Martin; Fuhrmann, Marine; Quere, Claudie; Goulitquer, Sophie; Corporeau, Charlotte; Renault, Tristan; Lapegue, Sylvie; Travers, Marie-agnes; Morga, Benjamin; Garriques, Manon; Garcia, Celine; Haffner, Philippe; Dubreuil, Christine; Faury, Nicole; Baillon, Laury; Baud, Jean-pierre. |
| Suite aux surmortalités observées au sein des élevages de moules touchant à la fois les animaux adultes et les juvéniles au mois de mars, dans les Pertuis Charentais, la Direction Générale de l’Alimentation (DGAl) a adressé une saisine à Ifremer le 04 avril 2014. Celle-ci fait état d’une demande d’appui scientifique et technique d’Ifremer pour tenter d’apporter des éléments de réponse par rapport à cette mortalité subite et importante. L’Ifremer a proposé un projet d’action permettant l’expertise du phénomène de surmortalité de la moule bleue, selon 3 axes : l’impact environnemental, la pathologie et les caractéristiques physiologiques des moules. Il s’agit de mettre en évidence la présence ou non d’agents infectieux connus ou émergents, d’apporter des... |
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| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00229/34022/32387.pdf |
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| Lapegue, Sylvie; Ben Salah, Inès; Batista, Frederico; Heurtebise, Serge; Neifar, Lassad; Boudry, Pierre. |
| Despite the economic importance of numerous oyster species throughout the world, our knowledge of their systematics and phylogeography remains fragmentary. In Europe, the indigenous species, Ostrea edulis, suffered overexploitation as well as two successive parasitosis over the last century. However, this species is still cultivated in Europe, and especially in Spain and France. Its distribution area stretches from the Atlantic coasts of Europe to the north of Africa as well as around the Mediterranean and Black Sea. Its Mediterranean and South Atlantic distribution area is shared with another indigenous species, the dwarf oyster, Ostreola stentina. Its very small adult size (a few centimetres) makes its culture pointless from a commercial point of view.... |
| Tipo: Text |
Palavras-chave: Ostreola stentina; Oyster; Taxonomy; Ostreola stentina; Huître; Taxonomie. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/acte-3364.pdf |
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| Bayne, B. L.; Ahrens, M.; Allen, S. K.; D'Auriac, M. Angles; Backeljau, T.; Beninger, P.; Bohn, R.; Boudry, Pierre; Davis, J.; Green, T.; Guo, X.; Hedgecock, D.; Ibarra, A.; Kingsley-smit, P.; Krause, M.; Langdon, C.; Lapegue, Sylvie; Li, C.; Manahan, D.; Mann, R.; Perez-paralle, L.; Powell, E. N.; Rawson, P. D.; Speiser, D.; Sanchez, J. -l.; Shumway, S.; Wang, H.. |
| Tipo: Text |
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| Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00418/52944/53886.pdf |
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| Batista, Frederico; Leitao, Alexandra; Huvet, Arnaud; Lapegue, Sylvie; Heurtebise, Serge; Boudry, Pierre. |
| The taxonomic status of the Portuguese oyster Crassostrea angulata and the Pacific oyster Crassostrea gigas has often been a matter of controversy. Based on larval shell morphology, experimental hybridisation and electrophoretic studies of enzyme polymorphism several authors have considered these two species as being synonymous. During the recent years, several genetic studies based on mitochondrial DNA and microsatellites data have provided evidences that the two taxa are genetically distinct although closely related. Furthermore, karyotype analyses have also highlighted the close genetic similarity of these taxa in comparison with other cupped oyster species. However, a comparative analysis of restriction enzymes ideograms revealed differences between... |
| Tipo: Text |
Palavras-chave: Geographical origin; Phylogenetic; Genetic; Taxonomic status; Crassostrea angulata; Oyster. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/acte-3433.pdf |
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| Lapegue, Sylvie; Heurtebise, Serge; Harrang, Estelle; Morga, Benjamin; Flahauw, Emilie; Sauvage, Christopher; Boudry, Pierre. |
| During the last five years, a great effort has been made to develop genomic tools in oyster species all over the world. In Europe, the Pacific cupped oyster, Crassostrea gigas, and the European flat oyster, Ostrea edulis received a special attention as they are the key species for local aquaculture. In particular, the Pacific oyster is a marine bivalve of major economical and ecological importance worldwide. Better knowledge of its physiology and its genetics contributes to optimize its aquaculture production using selective breeding or to understand its invasive behavior in some coastal areas. Current international developments of genomic resources include EST databases, BAC libraries, genome mapping and sequencing. The Genoscope (Evry, France) and the... |
| Tipo: Text |
Palavras-chave: Oysters; Crassostrea gigas; Ostrea edulis; Resistance pathogens; QTLs; Single Nucleotide Polymorphism. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00015/12584/9454.pdf |
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| Lapegue, Sylvie; Boutet, Isabelle; Leitao, Alexandra; Heurtebise, Serge; Garcia, P; Thiriot Quievreux, C; Boudry, Pierre. |
| Three species of man.-rove oysters, Crassostrea rhizophorae, C. brasiliana, and C. gasar, have been described along the Atlantic shores of South America and Africa. Because the distribution of these molluscs is of great biological and commercial interest, their taxonomy and distribution deserve further clarification. Therefore, 15 populations were sampled from both continents. Their 16S mitochondrial polymorphism was studied by sequencing and PCR-RFLP analysis. Two haplotypes were identified. Haplotype a was the only one observed in Africa, but it was also observed in South America together with haplotype b. Because C. gasar is the only mangrove oyster identified on the west coast of Africa, haplotype a was attributed to this species, which has thus been... |
| Tipo: Text |
Palavras-chave: Mangrove oyster; Crassostrea gasar; Karyotype; Phylogeography; 16S mitochondrial DNA. |
| Ano: 2002 |
URL: http://archimer.ifremer.fr/doc/2002/publication-2790.pdf |
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| Lallias, Delphine; Taris, Nicolas; Boudry, Pierre; Bonhomme, Francois; Lapegue, Sylvie. |
| In order to document further the phenomena of variance in reproductive success in natural populations of the European flat oyster Ostrea Midis, two complementary studies based on natural and experimental populations were conducted. The first part of this work was focused on paternity analyses using a set of four microsatellite markers for larvae collected from 13 brooding females sampled in Quiberon Bay (Brittany, France). The number of individuals contributing as the male parent to each progeny assay was highly variable, ranging from 2 to more than 40. Moreover, paternal contributions showed a much skewed distribution, with some males contributing to 50-100% of the progeny assay. The second part of this work consisted of the analysis of six successive... |
| Tipo: Text |
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| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00012/12329/9222.pdf |
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| Lallias, Delphine; Taris, Nicolas; Boudry, Pierre; Bonhomme, Francois; Lapegue, Sylvie. |
| he European flat oyster (Ostrea edulis L.) is a marine bivalve whose natural geographical distribution ranges along the European Atlantic coast from Norway to Morocco, in addition to the Mediterranean and Black Sea. Previous studies of allozymes, microsat-ellites and mitochondrial differentiation over the whole range concluded that a significant divergence existed between Mediterranean and Atlantic populations, together with an isolation-by-distance pattern. However, the average mitochondrial haplotypic diversity displayed a high among populations variance, reflecting smaller effective population size in some locations. Additionally, a ten-fold quantitative difference was observed in the same study in Fst between the mitochondrial and the nuclear genomes,... |
| Tipo: Text |
Palavras-chave: Genotypes; Genetic diversity; Breeding success; Allozymes; Population genetics; Parentage analyses; Natural population; Ostrea edulis; Oyster. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/acte-3334.pdf |
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| Arzul, Isabelle; Feist, Steve; Figueras, Antonio; Lapegue, Sylvie; Paillard, Christine; Furones, Dolors. |
| The European shellfish industry enjoys a privileged position on the global scene. Its social dimension is essential, as it employs a high number of people in more than 8000 companies, mostly micro-companies. Shellfish production in Europe is little diversified and mainly relies on the industrially produced mussels, oysters and clams. Over the recent years, this sector has grown more slowly than other fish farming sectors, notably because it depends a great deal on the environmental quality and the emergence of diseases. Mortality events, linked to pathogen organisms such as viruses, bacteria and parasites (protozoa), tend to weaken the production’s sustainability. In this context, the European project VIVALDI (PreVenting and mItigating farmed biVALve... |
| Tipo: Text |
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| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00352/46307/45975.pdf |
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| Registros recuperados: 140 | |
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